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mouse embryonic fibroblast retroviral packaging cell line gp e86  (ATCC)


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    ATCC mouse embryonic fibroblast retroviral packaging cell line gp e86
    Fig. 4 Insertion of the αN helix between MLL and AF6 disrupts myeloid immortalization and blocks its leukemogenic potential in mice. a Schematic of experimental strategies to measure immortalization of hematopoietic progenitors and induction of leukemogenesis. b Serial replating immortalization assay for progenitor cells transduced with <t>retroviral</t> constructs. Bars represent mean ± SD of the total colonies per 5 × 103 cells derived from three replicates. c Typical granulocyte and macrophage colonies from primary methylcellulose cultures of pMSCVneo or MLL- AF6αN-RA1 and macroscopic blast colonies from MLL-AF6NCR or MLL- AF6RA1 cultures. d Representative flow cytometry analysis of donor-derived myeloid cells in the bone marrow of transplanted mice. The red quadrants highlight the immature CD11b+GR1low/negative myeloid subpopulation which was significantly expanded in MLL-AF6NCR or MLL-AF6RA1 expressing bone marrow cells in primary transplants compared to control cells (vector) and MLL-AF6αN-RA1 expressing bone marrow cells. All mice transplanted with MLL-AF6NCR or MLL-AF6RA1 expressing cells were leukemic at 5 weeks ( > 20% blasts in the bone marrow and peripheral blood) while MLL- AF6αN-RA1 expressing cells did not induce leukemia up to 10 weeks after transplantation. e Donor-derived engraftment in the bone marrow of mice transplanted with 105 BM cells transduced with pMSCVneo, MLL-AF6NCR, MLL-AF6RA1 or MLL-AF6αN-RA1. BM cells were selected for 7 days in G418 prior to transplantation. Shown are the mean ± SD of donor cell engraftment (CD45.2 + ) and of the myeloid (CD11b+, Gr1+) to lymphoid (CD3+, B220+) ratio in donor-derived cells 5 weeks after transplantation (2 × 105/mice, n = 5 or 7 mice). Error bars represent s.d.
    Mouse Embryonic Fibroblast Retroviral Packaging Cell Line Gp E86, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblast+retroviral+packaging+cell+line+gp+e86/Tick+Cell+Line%2C+ISE6/pm29062045-286-36-46
    Average 95 stars, based on 184 article reviews
    mouse embryonic fibroblast retroviral packaging cell line gp e86 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Evolution of AF6-RAS association and its implications in mixed-lineage leukemia."

    Article Title: Evolution of AF6-RAS association and its implications in mixed-lineage leukemia.

    Journal: Nature communications

    doi: 10.1038/s41467-017-01326-5

    Fig. 4 Insertion of the αN helix between MLL and AF6 disrupts myeloid immortalization and blocks its leukemogenic potential in mice. a Schematic of experimental strategies to measure immortalization of hematopoietic progenitors and induction of leukemogenesis. b Serial replating immortalization assay for progenitor cells transduced with retroviral constructs. Bars represent mean ± SD of the total colonies per 5 × 103 cells derived from three replicates. c Typical granulocyte and macrophage colonies from primary methylcellulose cultures of pMSCVneo or MLL- AF6αN-RA1 and macroscopic blast colonies from MLL-AF6NCR or MLL- AF6RA1 cultures. d Representative flow cytometry analysis of donor-derived myeloid cells in the bone marrow of transplanted mice. The red quadrants highlight the immature CD11b+GR1low/negative myeloid subpopulation which was significantly expanded in MLL-AF6NCR or MLL-AF6RA1 expressing bone marrow cells in primary transplants compared to control cells (vector) and MLL-AF6αN-RA1 expressing bone marrow cells. All mice transplanted with MLL-AF6NCR or MLL-AF6RA1 expressing cells were leukemic at 5 weeks ( > 20% blasts in the bone marrow and peripheral blood) while MLL- AF6αN-RA1 expressing cells did not induce leukemia up to 10 weeks after transplantation. e Donor-derived engraftment in the bone marrow of mice transplanted with 105 BM cells transduced with pMSCVneo, MLL-AF6NCR, MLL-AF6RA1 or MLL-AF6αN-RA1. BM cells were selected for 7 days in G418 prior to transplantation. Shown are the mean ± SD of donor cell engraftment (CD45.2 + ) and of the myeloid (CD11b+, Gr1+) to lymphoid (CD3+, B220+) ratio in donor-derived cells 5 weeks after transplantation (2 × 105/mice, n = 5 or 7 mice). Error bars represent s.d.
    Figure Legend Snippet: Fig. 4 Insertion of the αN helix between MLL and AF6 disrupts myeloid immortalization and blocks its leukemogenic potential in mice. a Schematic of experimental strategies to measure immortalization of hematopoietic progenitors and induction of leukemogenesis. b Serial replating immortalization assay for progenitor cells transduced with retroviral constructs. Bars represent mean ± SD of the total colonies per 5 × 103 cells derived from three replicates. c Typical granulocyte and macrophage colonies from primary methylcellulose cultures of pMSCVneo or MLL- AF6αN-RA1 and macroscopic blast colonies from MLL-AF6NCR or MLL- AF6RA1 cultures. d Representative flow cytometry analysis of donor-derived myeloid cells in the bone marrow of transplanted mice. The red quadrants highlight the immature CD11b+GR1low/negative myeloid subpopulation which was significantly expanded in MLL-AF6NCR or MLL-AF6RA1 expressing bone marrow cells in primary transplants compared to control cells (vector) and MLL-AF6αN-RA1 expressing bone marrow cells. All mice transplanted with MLL-AF6NCR or MLL-AF6RA1 expressing cells were leukemic at 5 weeks ( > 20% blasts in the bone marrow and peripheral blood) while MLL- AF6αN-RA1 expressing cells did not induce leukemia up to 10 weeks after transplantation. e Donor-derived engraftment in the bone marrow of mice transplanted with 105 BM cells transduced with pMSCVneo, MLL-AF6NCR, MLL-AF6RA1 or MLL-AF6αN-RA1. BM cells were selected for 7 days in G418 prior to transplantation. Shown are the mean ± SD of donor cell engraftment (CD45.2 + ) and of the myeloid (CD11b+, Gr1+) to lymphoid (CD3+, B220+) ratio in donor-derived cells 5 weeks after transplantation (2 × 105/mice, n = 5 or 7 mice). Error bars represent s.d.

    Techniques Used: Transduction, Retroviral, Construct, Derivative Assay, Cytometry, Expressing, Control, Plasmid Preparation, Transplantation Assay



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    mouse embryonic fibroblast retroviral packaging cell line gp e86  (ATCC)
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    ATCC mouse embryonic fibroblast retroviral packaging cell line gp e86
    Fig. 4 Insertion of the αN helix between MLL and AF6 disrupts myeloid immortalization and blocks its leukemogenic potential in mice. a Schematic of experimental strategies to measure immortalization of hematopoietic progenitors and induction of leukemogenesis. b Serial replating immortalization assay for progenitor cells transduced with <t>retroviral</t> constructs. Bars represent mean ± SD of the total colonies per 5 × 103 cells derived from three replicates. c Typical granulocyte and macrophage colonies from primary methylcellulose cultures of pMSCVneo or MLL- AF6αN-RA1 and macroscopic blast colonies from MLL-AF6NCR or MLL- AF6RA1 cultures. d Representative flow cytometry analysis of donor-derived myeloid cells in the bone marrow of transplanted mice. The red quadrants highlight the immature CD11b+GR1low/negative myeloid subpopulation which was significantly expanded in MLL-AF6NCR or MLL-AF6RA1 expressing bone marrow cells in primary transplants compared to control cells (vector) and MLL-AF6αN-RA1 expressing bone marrow cells. All mice transplanted with MLL-AF6NCR or MLL-AF6RA1 expressing cells were leukemic at 5 weeks ( > 20% blasts in the bone marrow and peripheral blood) while MLL- AF6αN-RA1 expressing cells did not induce leukemia up to 10 weeks after transplantation. e Donor-derived engraftment in the bone marrow of mice transplanted with 105 BM cells transduced with pMSCVneo, MLL-AF6NCR, MLL-AF6RA1 or MLL-AF6αN-RA1. BM cells were selected for 7 days in G418 prior to transplantation. Shown are the mean ± SD of donor cell engraftment (CD45.2 + ) and of the myeloid (CD11b+, Gr1+) to lymphoid (CD3+, B220+) ratio in donor-derived cells 5 weeks after transplantation (2 × 105/mice, n = 5 or 7 mice). Error bars represent s.d.
    Mouse Embryonic Fibroblast Retroviral Packaging Cell Line Gp E86, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblast+retroviral+packaging+cell+line+gp+e86/Tick+Cell+Line%2C+ISE6/pm29062045-286-36-46
    Average 95 stars, based on 1 article reviews
    mouse embryonic fibroblast retroviral packaging cell line gp e86 - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

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    Fig. 4 Insertion of the αN helix between MLL and AF6 disrupts myeloid immortalization and blocks its leukemogenic potential in mice. a Schematic of experimental strategies to measure immortalization of hematopoietic progenitors and induction of leukemogenesis. b Serial replating immortalization assay for progenitor cells transduced with retroviral constructs. Bars represent mean ± SD of the total colonies per 5 × 103 cells derived from three replicates. c Typical granulocyte and macrophage colonies from primary methylcellulose cultures of pMSCVneo or MLL- AF6αN-RA1 and macroscopic blast colonies from MLL-AF6NCR or MLL- AF6RA1 cultures. d Representative flow cytometry analysis of donor-derived myeloid cells in the bone marrow of transplanted mice. The red quadrants highlight the immature CD11b+GR1low/negative myeloid subpopulation which was significantly expanded in MLL-AF6NCR or MLL-AF6RA1 expressing bone marrow cells in primary transplants compared to control cells (vector) and MLL-AF6αN-RA1 expressing bone marrow cells. All mice transplanted with MLL-AF6NCR or MLL-AF6RA1 expressing cells were leukemic at 5 weeks ( > 20% blasts in the bone marrow and peripheral blood) while MLL- AF6αN-RA1 expressing cells did not induce leukemia up to 10 weeks after transplantation. e Donor-derived engraftment in the bone marrow of mice transplanted with 105 BM cells transduced with pMSCVneo, MLL-AF6NCR, MLL-AF6RA1 or MLL-AF6αN-RA1. BM cells were selected for 7 days in G418 prior to transplantation. Shown are the mean ± SD of donor cell engraftment (CD45.2 + ) and of the myeloid (CD11b+, Gr1+) to lymphoid (CD3+, B220+) ratio in donor-derived cells 5 weeks after transplantation (2 × 105/mice, n = 5 or 7 mice). Error bars represent s.d.

    Journal: Nature communications

    Article Title: Evolution of AF6-RAS association and its implications in mixed-lineage leukemia.

    doi: 10.1038/s41467-017-01326-5

    Figure Lengend Snippet: Fig. 4 Insertion of the αN helix between MLL and AF6 disrupts myeloid immortalization and blocks its leukemogenic potential in mice. a Schematic of experimental strategies to measure immortalization of hematopoietic progenitors and induction of leukemogenesis. b Serial replating immortalization assay for progenitor cells transduced with retroviral constructs. Bars represent mean ± SD of the total colonies per 5 × 103 cells derived from three replicates. c Typical granulocyte and macrophage colonies from primary methylcellulose cultures of pMSCVneo or MLL- AF6αN-RA1 and macroscopic blast colonies from MLL-AF6NCR or MLL- AF6RA1 cultures. d Representative flow cytometry analysis of donor-derived myeloid cells in the bone marrow of transplanted mice. The red quadrants highlight the immature CD11b+GR1low/negative myeloid subpopulation which was significantly expanded in MLL-AF6NCR or MLL-AF6RA1 expressing bone marrow cells in primary transplants compared to control cells (vector) and MLL-AF6αN-RA1 expressing bone marrow cells. All mice transplanted with MLL-AF6NCR or MLL-AF6RA1 expressing cells were leukemic at 5 weeks ( > 20% blasts in the bone marrow and peripheral blood) while MLL- AF6αN-RA1 expressing cells did not induce leukemia up to 10 weeks after transplantation. e Donor-derived engraftment in the bone marrow of mice transplanted with 105 BM cells transduced with pMSCVneo, MLL-AF6NCR, MLL-AF6RA1 or MLL-AF6αN-RA1. BM cells were selected for 7 days in G418 prior to transplantation. Shown are the mean ± SD of donor cell engraftment (CD45.2 + ) and of the myeloid (CD11b+, Gr1+) to lymphoid (CD3+, B220+) ratio in donor-derived cells 5 weeks after transplantation (2 × 105/mice, n = 5 or 7 mice). Error bars represent s.d.

    Article Snippet: For gene transfer into bone marrow cells, pMSCV constructs consisting of in-frame fusions between MLL and various protein partners (MSCV, -FLAG-AF6NCR, -MLL-AF6NCR, -MLL-AF6RA1, -MLLAF6αN-RA1) were used to produce high-titer, helper-free recombinant retroviruses by transduction of the mouse embryonic fibroblast retroviral packaging cell line GP + E86 (ATCC CRL-9642) with VSV-G-pseudotyped MSCV particles72.

    Techniques: Transduction, Retroviral, Construct, Derivative Assay, Cytometry, Expressing, Control, Plasmid Preparation, Transplantation Assay